Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit提供了一種熒光分析方法，用于檢測廣泛生物靶標的細胞內鈣動員情況。相對于其他商業化的染料，GFP-CERTIFIED® FLUOFORTE®染料是最亮和最-靈-敏的熒光鈣指示劑。該試劑盒為貼壁和非貼壁細胞系提供了更少步驟，更低變異性，更簡易的鈣檢測。此外，它既不需要洗滌步驟，也不需要添加外源性淬滅染料，避免了對受體-配體相互作用動力學的干擾。
Enzo Life Sciences的GFP-CERTIFIED® FLUOFORTE® Calcium assay kit可用于細胞內鈣離子的高通量檢測，鈣離子是重要的第二信使，常用于初步篩選中監測G蛋白偶聯受體激活。
產品特色
● 可監測表達GFP細胞中的鈣離子動員情況
● 最明亮、最-靈-敏的熒光Ca2+指示劑
● 獲得的 EC50和IC50值與使用鈣離子熒光探針 Rhod-4 AM和Fluo-4 AM 相當
● 更大范圍的檢測窗口
● 使用方便，無需洗滌，無需淬滅染料
實驗示例
圖2. U-2 OS細胞分別與（A）GFP-CERTIFIED® FLUOFORTE®AM染料和（B）Rhod-2 AM染料孵育。洗滌細胞并用終濃度為100nM的ATP處理，并立即成像。GFP-CERTIFIED® FLUOFORTE® AM染料比Rhod-2 AM表現出更亮的熒光強度。
產品信息
產品貨號 | ENZ-51020-KP010/ ENZ-51020-KP100 |
產品名稱 | GFP-CERTIFIED® FLUOFORTE® Calcium assay kit |
規 格 | 10*96wells/100*96wells |
短期保存 | -20°C |
長期保存 | -80°C |
試劑盒組分 | Starter pack (Prod. No. ENZ-51020-KP010): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 1 vial Reagent B (dye efflux inhibitor), 10ml Reagent C (Hanks’ buffer with 20mM HEPES, 100ml) Sufficient for 10 x 96-well plates High-throughput (Prod. No. ENZ-51020-KP100): Reagent A (lyophilized GFP-CERTIFIED® FLUOFORTE® dye), 10 vials Reagent B (dye efflux inhibitor), 10 x 10ml Sufficient for 100 x 96-well plates |
部分產品引用文獻
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5. Interplay between sub-cellular alterations of calcium release and T-tubular defects in cardiac diseases: M. Scardigli, et al.; Front. Physiol. 9, 1474 (2018)
6. Characterization of cholesterol homeostasis in sphingosine-1-phosphate lyase-deficient fibroblasts reveals a Niemann-Pick disease type C-like phenotype: H. Vienken, et al.; Sci. Rep. 7, 43575 (2017)
7. Novel insights on the relationship between T-tubular defects and contractile dysfunction in a mouse model of hypertrophic cardiomyopathy: C. Crocini, et al.; J. Mol. Cell. Cardiol. 91, 42 (2016)
8. Sulfur dioxide protects against collagen accumulation in pulmonary artery in association with downregulation of the transforming growth factor β1/smad pathway in pulmonary hypertensive rats: W. Yu, et al.; J. Am. Heart Assoc. 5, e003910 (2016)
9. T-tubular electrical defects contribute to blunted β-adrenergic response in heart failure: C. Crocini, et al.; Int. J. Mol. Sci. 17, 1471 (2016)
10. Defects in T-tubular electrical activity underlie local alterations of calcium release in heart failure: C. Crocini, et al.; PNAS 111, 15196 (2014)
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